Adenosine Triphosphate: Guanosine Monophosphate Phosphotransferase

Adenosine Triphosphate : Guanosine Monophosphate Phosphotransferase

Several laboratories have reported studies on individual enzymes that belong to the general class of adenosine triphosphate : nucleoside monophosphate phosphotransferases (EC 2.7.4.4), commonly termed nucleoside monophosphate kinases (l-7). Studies with partially purified enzyme preparations have indicated that a variety of nucleoside monophosphates can serve as alternative substrates for nucle...

Adenosine triphosphate: adenosine monophosphate phosphotransferase isozymes in rat liver and hepatomas.

Rat liver adenosine triphosphate: adenosine monophosphate phosphotransferase activity. II. Subcellular localization of adenylate kinase isozymes.

Adenylate kinase activity (ATP:AMP-phosphotransferase, EC 2.7.4.3) was studied in subcellular extracts of rat liver tissue. Essentially all of the activity, over 150 units per g of tissue, wet weight (1.7 units per mg of protein), was released into the cytosol when the tissue was homogenized in 0.025 M sucrose. Isotonic (0.25 M sucrose) homogenization and separation of subcellular fractions by ...

Nonenzymatic formation of guanosine 3':5'-monophosphate from guanosine triphosphate.

We observed the nonenzymatic formation of guanosine 3’:5’-monophosphate (cyclic GMP) from GTP. The reaction was dependent upon heat and was accelerated by divalent cations and creatine phosphate. The formation of cyclic GMP was greater with increased incubation time, temperature, GTP concentration, and pH. All of the divalent cations tested (Mg2+, Mnz+, Ca2+, Ba2+, and Znzf) increased cyclic GM...

The purification and properties of adenosine triphosphate-lombricine phosphotransferase.

Gundlach, H. G., Stein, W. H. & Moore, S. (1959). J. biol. Chem. 234, 1754. Kratochvil, C. H. & Deutsch, H. F. (1956). J. biol. Chem. 222, 31. Leder, P. (1962). Nature, Lond., 193, 1087. Oncley, J. L., Scatchard, G. & Brown, A. (1947). J. phy8. Chem. 51, 184. Overbeek, J. Th. G. (1950). Advanc. CoUoid Sci. 3, 97. Spackman, D. H., Stein, W. H. & Moore, S. (1958). Analyt. Chem. 30, 1190. Tanford,...